Cell culture material

FCeM^® Advance-CR

The FCeM^® Advance-CR Preparation Kit (containing FP003B polymer) not only makes it possible to achieve the function of cell dispersion and suspension culture without agitation but also provides high efficiency of cell recovering from the 3D cell culture suspension using our proprietary Harvesting Buffer followed by centrifugation.

Details are here

SPHEROIDS / ORGANOIDS CULTURE

Spheroids in FCeM^® Advance-CR medium	Conventional gel culture Hydrogel culture is one of the most widely used methods for spheroids or organoids culture in vitro. In general, gel matrix materials are known as containing growth factors from natural tissues. Due to its natures, the following issues may be problematic. Formation of hydrogel in culture medium requires temperature control on ice. After gel culture, spheroids or organoids have to be collected from the hydrogel. Identification of effective growth factor from gel matrix is generally difficult. Application of hydrogels for clinical use is restricted because of the existence of animal derived materials . Why Choose FCeM^® Advance-CR – 3D Culture Viscosity of culture medium will not be changed! Perform pipetting mix or dispensing of medium freely. Unwanted aggregation between cell spheroids or organoids will be prevented! Temperature control is not necessary! Preparation of FCeM^® Advance-CR for clinical use such as transplantation is now ongoing. If you aim at medical application, please contact us beforehand.

Spheroids in FCeM^®
Advance-CR medium

Conventional gel culture

Hydrogel culture is one of the most widely used methods for spheroids or organoids culture in vitro. In general, gel matrix materials are known as containing growth factors from natural tissues. Due to its natures, the following issues may be problematic.

Formation of hydrogel in culture medium requires temperature control on ice.
After gel culture, spheroids or organoids have to be collected from the hydrogel.
Identification of effective growth factor from gel matrix is generally difficult.
Application of hydrogels for clinical use is restricted because of the existence of animal derived materials .

Why Choose FCeM^® Advance-CR – 3D Culture

Viscosity of culture medium will not be changed!
Perform pipetting mix or dispensing of medium freely.
Unwanted aggregation between cell spheroids or organoids will be prevented!
Temperature control is not necessary!

Preparation of FCeM^® Advance-CR for clinical use such as transplantation is now ongoing. If you aim at medical application, please contact us beforehand.

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MSC SPHEROIDS PRESERVATION AT ROOM TEMPERAYURE

Spheroids of mesenchymal stem cells suspended in FP003B-containing medium were stored statically in sealed conical tubes under room temperature and maintained high viability after 10 days. It indicates the possibility to apply FP003B for non-crypreservation of spheroids for long distance transportation and therapy.

Publications

Torizal, F.G., Lau, Q.Y., Ibuki, M. et al. A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation. Commun Biol 4, 1316 (2021). (Impact Factor: 6.268)
Furuhata Y, Sakai A, Murakami T, Nagasaki A, Kato Y. Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. PLoS One. 2020 Jan 3;15(1). (Impact Factor: 2.740)
Horiguchi, I, Torizal, FG, Nagate, H, et al. Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid. Biotechnol Progress. 2020. (Impact Factor: 2.334)
Fuad Torizal, Qiao You Lau, Masato Ibuki et al. High-density hiPSCs expansion supported by growth factors accumulation in a simple dialysis-culture platform, 09 October 2020, PREPRINT (Version 1) available at Research Square.
Chen YC, Chang WC, Lin SP, et al. Rival-Gervier S, Kanaki T, Wu SC, Pain B. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS One. 2018 Sep 21;13(9). (Impact Factor: 2.740)
Hiroki Okumura, Eri Nanizawa, Anna Nakanishi, et al. Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging. Adv. Biosys. 2018, 2, 1800137. (Impact Factor: 3.536)