Cell culture material

FCeM^® Advance Preparation Kit

The FCeM^® Advance Preparation Kit (containing FP003 polymer) not only makes it possible to achieve the function of cell dispersion and suspension culture without agitation but also provides high efficiency of cell recovering from the 3D cell culture suspension using our proprietary Harvesting Buffer followed by centrifugation.

SPHEROIDS / ORGANOIDS CULTURE

Spheroids in FCeM^® Advance medium	Conventional gel culture Hydrogel culture is one of the most widely used methods for spheroids or organoids culture in vitro. In general, gel matrix materials are known as containing growth factors from natural tissues. Due to its natures, the following issues may be problematic. Formation of hydrogel in culture medium requires temperature control on ice. After gel culture, spheroids or organoids have to be collected from the hydrogel. Identification of effective growth factor from gel matrix is generally difficult. Application of hydrogels for clinical use is restricted because of the existence of animal derived materials . Why Choose FCeM® Advance – 3D Culture Viscosity of culture medium will not be changed! Perform pipetting mix or dispensing of medium freely. Unwanted aggregation between cell spheroids or organoids will be prevented! Temperature control is not necessary! Preparation of FCeM Advance for clinical use such as transplantation is now ongoing. If you aim at medical application, please contact us beforehand.

Spheroids in FCeM^®
Advance medium

Conventional gel culture

Hydrogel culture is one of the most widely used methods for spheroids or organoids culture in vitro. In general, gel matrix materials are known as containing growth factors from natural tissues. Due to its natures, the following issues may be problematic.

Formation of hydrogel in culture medium requires temperature control on ice.
After gel culture, spheroids or organoids have to be collected from the hydrogel.
Identification of effective growth factor from gel matrix is generally difficult.
Application of hydrogels for clinical use is restricted because of the existence of animal derived materials .

Why Choose FCeM® Advance – 3D Culture

Viscosity of culture medium will not be changed!
Perform pipetting mix or dispensing of medium freely.
Unwanted aggregation between cell spheroids or organoids will be prevented!
Temperature control is not necessary!

Preparation of FCeM Advance for clinical use such as transplantation is now ongoing. If you aim at medical application, please contact us beforehand.

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MSC SPHEROIDS PRESERVATION AT ROOM TEMPERAYURE

Spheroids of mesenchymal stem cells suspended in FP003-containing medium were stored statically in sealed conical tubes under room temperature and maintained high viability after 10 days. It indicates the possibility to apply FP003 for non-crypreservation of spheroids for long distance transportation and therapy.

Line-up and Protocols

383-10111	FCeM^® Advance Preparation Kit
Protocol	Media Preparation	EN
		JP
		CN
	3D Expansion Culture	EN
	3D Expansion Culture	JP
	3D Differentiation of iPSC Cells	Please Contact Us
	Non-Freezing 3D Reservation	Please Contact Us
387-12951	FCeM^® Advance Preparation Kit500
Protocol	Media Preparation	EN
		JP
		CN
	3D Expansion Culture	EN
	3D Expansion Culture	JP
	3D Differentiation of iPSC Cells	Please Contact Us
	Non-Freezing 3D Reservation	Please Contact Us

Publications

Torizal, F.G., Lau, Q.Y., Ibuki, M. et al. A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation. Commun Biol 4, 1316 (2021). (Impact Factor: 6.268)
Furuhata Y, Sakai A, Murakami T, Nagasaki A, Kato Y. Bioluminescent imaging of Arabidopsis thaliana using an enhanced Nano-lantern luminescence reporter system. PLoS One. 2020 Jan 3;15(1). (Impact Factor: 2.740)
Horiguchi, I, Torizal, FG, Nagate, H, et al. Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid. Biotechnol Progress. 2020. (Impact Factor: 2.334)
Fuad Torizal, Qiao You Lau, Masato Ibuki et al. High-density hiPSCs expansion supported by growth factors accumulation in a simple dialysis-culture platform, 09 October 2020, PREPRINT (Version 1) available at Research Square.
Chen YC, Chang WC, Lin SP, et al. Rival-Gervier S, Kanaki T, Wu SC, Pain B. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS One. 2018 Sep 21;13(9). (Impact Factor: 2.740)
Hiroki Okumura, Eri Nanizawa, Anna Nakanishi, et al. Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging. Adv. Biosys. 2018, 2, 1800137. (Impact Factor: 3.536)